As a result of these properties, the MSCs have great clinical application prospects, especially in the regeneration of injured tissues, functional reconstruction, and cell therapy. Blood 107, 1878–1887 (2006).Mesenchymal stem cells (MSCs) are multipotent cells that show self-renewal, multi-directional differentiation, and paracrine and immune regulation. Reconstitution of the functional human hematopoietic microenvironment derived from human mesenchymal stem cells in the murine bone marrow compartment. Characterization and purification of osteogenic cells from murine bone marrow by two-color cell sorting using anti-Sca-1 monoclonal antibody and wheat germ agglutinin. Van Vlasselaer, P., Falla, N., Snoeck, H. Isolation murine mesenchymal stem cells by positive selection. Characterization of a 5-fluorouracil-enriched osteoprogenitor population of the murine bone marrow. Characterization of mesenchymal stem cells isolated from murine bone marrow by negative selection. Purified mesenchymal stem cells are an efficient source for iPS cell induction. The elusive nature and function of mesenchymal stem cells. Prospective identification, isolation, and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow. Development of mesenchymal stem cells partially originate from the neural crest. Two distinct stem cell lineages in murine bone marrow. In search of the in vivo identity of mesenchymal stem cells. Primary murine MSC show highly efficient homing to the bone marrow but lose homing ability following culture. Replicative senescence of mesenchymal stem cells: a continuous and organized process. A protocol for isolation and culture of mesenchymal stem cells from mouse compact bone. A protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow. Adult stem cells from bone marrow (MSCs) isolated from different strains of inbred mice vary in surface epitopes, rates of proliferation, and differentiation potential. Peister, A., Mellad, J.A., Larson, B.L., Hall, B.M. Plastic adherent stromal cells from the bone marrow of commonly used strains of inbred mice: variations in yield, growth, and differentiation. Bone marrow stromal cells attenuate sepsis via prostaglandin E(2)-dependent reprogramming of host macrophages to increase their interleukin-10 production. Mesenchymal stem cell-mediated immunosuppression occurs via concerted action of chemokines and nitric oxide. Mesenchymal stem cells in health and disease. The role of stromal stem cells in tissue regeneration and wound repair. The development of fibroblast colonies in monolayer cultures of guinea-pig bone marrow and spleen cells. Self-renewing osteoprogenitors in bone marrow sinusoids can organize a hematopoietic microenvironment. The total protocol takes ∼7 h to complete.Ĭaplan, A.I. Approximately 10,000 PαS MSCs may then be isolated per mouse. ![]() Appropriate gates are constructed on a cell sorter to exclude dead cells and lineage (CD45 +Ter-119 +)-positive cells. The cell suspension is filtered before red blood cell lysis and incubated with the following antibodies: allophycocyanin (APC)-conjugated PDGFR-α, FITC-conjugated Sca-1, phycoerythrin (PE)-conjugated CD45 and Ter119. The crushed bones are then chopped and incubated for 1 h at 37 ☌ in 20 ml of DMEM containing 0.2% (wt/vol) collagenase. In brief, the tibia and femora are isolated and crushed using a pestle and mortar. Here we describe in detail our protocol to isolate PαS MSCs using flow cytometry. In addition, the selective isolation of PαS MSCs avoids cellular contamination that can complicate other methods. PDGFR-α +Sca-1 + (PαS) MSCs have augmented growth potential and robust tri-lineage differentiation compared with standard culture-selected MSCs. Platelet-derived growth factor receptor α (PDGFR-α) and stem cell antigen 1 (Sca-1) have recently been identified as selective markers of mouse mesenchymal stem cells (MSCs).
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